Imaging Cell Migration in Xenopus | @VirajDoddihal

Microscopy image of Xenopus embryo. The cellular skeletal protein actin (grey) marks the cell edges and the nucleus is in magenta. Credit: Viraj Doddihal

During their time in the 美女直播做爱鈥檚 2021 Embryology course, graduate students聽, the Stowers Institute, and聽, University of Southern California, spent a lot of time looking under the microscope.

That time paid off, and, along with some聽other stunning images, the pair captured this video of cell migration in a small tissue sample of a聽Xenopus laevis听别尘产谤测辞.

The cellular skeletal protein actin (grey) marks the cell edges and the nucleus is in magenta.

鈥淭his tissue behaves similar to how it would in an embryo,鈥 said Doddihal.

In the video, the cells migrate towards the top right, and during a process called 鈥渃onvergent extension,鈥 the cells intercalate to form a long, thin structure.

鈥淭his process plays a major role in turning a spherical frog embryo into a lengthened tadpole,鈥 said Doddihal.

This movie was captured on Zeiss 780 during the 2021 Embryology course.

The video was聽聽with Komatsu and Doddihal both listed as first authors. Embryology faculty member Chenbei Chang, University of Alabama at Birmingham, also contributed to the paper.

Citation:

Komatsu, V., Doddihal, V., Chang, C. (2021). Imaging of dynamic actin remodeling reveals distinct behaviors of head and trunk mesoderm in gastrulating Xenopus laevis. microPublication Biology. DOI:聽.