Back to Resources & Research Facilities The National Xenopus Resource Overview MBLthe NXR Ordering and Pricing Animal Care Mutant Frog Stocks Transgenic Tropicalis Transgenic Laevis Wild Type Frog Stocks Research Facility Resource Workshops and Meetings Contact The NXR Home Groundbreaking Research at the 美女直播做爱 Resources & Research Facilities The National Xenopus Resource Transgenic Tropicalis Xtr.Et(CAGGS-SB10;cryga:RFP)622EMead NXR_1002 Description: (622EHoppers)听SB10 transposase expression from CAGGS promoter/enhancer, 纬-cry driving RFP. 622E donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)7M8FMead NXR_1003 Description: (7M8FHoppers)听SB10 transposase expression from CAGGS promoter/enhancer, 纬-cry driving RFP. 7M and 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)7MMead NXR_1004 Description: (7MHoppers)听SB10 transposase expression from CAGGS promoter/enhancer, 纬-cry driving RFP. 7M donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)8FMead NXR_1005 Description: (8FHoppers)听SB10 transposase expression from CAGGS promoter/enhancer, 纬-cry driving RFP. 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Tg(CMV:memGFP)NXR NXR_1007 Description:听(CMVmemGFP) CMV driving ubiquitous expression of membrane GFP Reference: Custom line听generated by the NXR. Xtr.Tg(eef1a1:GFP)Krieg NXR_1008 Description:听(Ef1a-GFP) Eef1a1 driving GFP. Widespread GFP expression apparent from mid-gastrula on. 搁别蹿别谤别苍肠别:听听 Xtr.Et(gata1:GFP)mysMead NXR_1012 Description: (mTol2G1GFP-M4msy) GATA-1 promoter driving GFP, mister stripey integration into the apelin receptor gene. GFP expression in red blood cells and venous vasculature. Reference: Mead Lab;听听.听 Xtr.Et(krt8:GAL4-VP16,UAS:GFP)Mead NXR_1014 Description: (mTol2k8GAL4 VP16 UASGFP) Keratin 8 minimal promoter driving expression of GAL4VP16 fusion with UAS GFP reporter gene. Made using the miniTol2 transposon. Reference: Mead Lab;听听 Xtr.Tg(otx2:GFP)Kellr NXR_1020 Description: (Otx2-GFP) Otx2 driving GFP. GFP expression of forebrain and midbrain 搁别蹿别谤别苍肠别:听听听听 Xtr.Tg(pax6:GFP;cryga:RFP;actc1:RFP)Grngr NXR_1021 Description: (Pax6-GFP:xcryFRP:CardiacActin-RFP) Coinjection of pax6 driving GFP, 纬-cry driving RFP, and actc1 driving RFP. GFP expression in eyes, brain, and spinal cord. RFP expression in lenses and muscle. 搁别蹿别谤别苍肠别:听听 Xtr.Et(CAGGS-SB10;cryga:RFP; CAGGS:GFP)622EMead NXR_1025 Description:听(pT2BGFP-622E) Widespread GFP fluorescence: outcross of the 鈥榟opper鈥 frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression. Reference: Mead Lab;听听听 Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)6MMead NXR_1026 Description: (pT2BGFP-6M) Widespread GFP fluorescence. Reference: Mead Lab;听听听 Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)8FMead NXR_1028 Description: (pT2BGFP-8F) LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Lab;听听听 Xtr.Et(CAGGS-SB10;cryga:RFP)Mead NXR_1034 Description: (SB10enzyme) SB10 transposase expression from CAGGS promoter/enhancer,听纬-cry driving RFP. Reference: Mead Xtr.Et(eef1a1:GFP)Hbr,Cen,Fol,BruMead NXR_1037 Description: (Tol2X1G-12MHbrCenFolBru)听Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Reference: Mead Xtr.Et(eef1a1:GFP)folMead NXR_1038 Description: (Tol2X1G-12Mfol)听Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Fol integration into 5鈥 UTR of MTHFD1L gene. 搁别蹿别谤别苍肠别:听 Mead Lab; 听听听 Xtr.Et(eef1a1:GFP)cenMead NXR_1040 Description:听(Tol2X1G-Mcen) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Cen expressed in forebrain. Reference: Mead Lab; 听听听 Xtr.Et(eef1a1:GFP)grbMead NXR_1042 Description:听听(Tol2X1G-12Mgrb) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP.听 Garibaldi (grb) integration into 3鈥 UTR of smad7 gene. Reference: Mead Lab; 听听听 Xtr.Et(eef1a1:GFP)hbrMead NXR_1043 Description:听(Tol2X1G-12Mhbr) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Expression in cartilage and cardiac outflow tract. Reference: Mead Lab; 听听听 Xtr.Et(eef1a1:GFP)johMead NXR_1044 Description: (Tol2X1G-12Mjoh) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Joh expressed in kidney, integration near HNF1B gene. 搁别蹿别谤别苍肠别:听 Mead Lab; 听听听 Xtr.Et(eef1a1:GFP)psvbiMead NXR_1046 Description: (Tol2X1G-16Fpsvbi)听Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Medial somite and ventral blood island expression. Integration likely to be in repeart regiong of MLL2 gene. Reference: Mead听 Xtr.Et(eef1a1:GFP)16FMead NXR_1047 Description:听(Tol2X1G-16Fubiq) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. 16F fubiq Reference: Mead Lab; 听听听 Xtr.Tg(cryga:GFP)Grngr NXR_1049 Description: (xCrys-GFP) GFP expression in the lens. Reference: Grainger.听 Xtr.Tg(CMV:Aequorin,GFP)NXR NXR_1070 Description: (CMV-Aequorin-GFP) Reference: Custom line generated听by NXR for Andrew Miller. Xtr.Tg(tubb2b:GFP)Amaya NXR_1092 Description: (NBT/Tubb2-GFP) N-tubulin promoter driving GFP. GFP expression in neural tissue. 搁别蹿别谤别苍肠别:听听Xenbase. Xtr.Tg(vim:Venus;cryga:GFP)Amaya NXR_1093 Description: (Vimentin-GFP) Vimentin promoter driving Venus GFP, 纬-cry driving GFP marker. GFP expression labels the deep cells of the neurectoderm and neural progenitors. GFP expression in lens. 搁别蹿别谤别苍肠别:听听Xenbase. Xtr.Tg(WntREs:dEGFP)Vlemx NXR_1094 Description: (pbin7LEF-GFP/Wnt Reporter)听GFP expression reveals dynamic patterns of Wnt/beta-catenin signaling activity. The inserted transgene construct contains a synthetic Wnt-responsive promoter consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized eGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. 搁别蹿别谤别苍肠别:听 Xtr.Tg(pax6:PR-Gal4-VP16;cryga:eCFP)Zimml NXR_1098 Description:听(Pax6galPRBicolorBlue)听Pax6 promoter driving RU486-inducible progesterone receptor/VP16/Gal4 transactivator, in transgene marked by g-cry ECFP cassette.听ECFP in lens of eye; inducible Gal4 drives expression of UAS-transgenes in the presence of RU486 in the eye and dorsal CNS.听Labels lens of eye; other functions depend on separate effector UAS transgenes 搁别蹿别谤别苍肠别:听听 Xtr.Tg(tubb2b:Hsa.ALPP;cryga:dsRed)Amaya NXR_1099 Description: (NBT-PLAPxcryRFP) Neural expression of human placental alkaline phosphatase (ALPP) driven by N-beta-tubulin (tubb2b) upstream region. dsRed expression in eye from gamma crystallin (cryga) promoter used as marker. 搁别蹿别谤别苍肠别:听听.听 Xtr.Tg(Hsa.UBC.:lyn-memVenus)Amaya NXR_1106 Description: (UbiC-membraneVenus) Human Ubiquitin C (Hsa.UBC) promoter driving expression of a lyn kinase membrane domain fused to venus GFP. Ubiquitous Venus expression of cell membrane. 搁别蹿别谤别苍肠别:听.听 Xtr.Tg(pax6:GFP)Amaya NXR_1107 Description: (Pax6-GFP) 3.6kb PAX6 promoter driving GFP. GFP expression in anterior and posterior neural plate plate. 搁别蹿别谤别苍肠别:听.听 Xtr.Tg(UbiC Flox RFPGFP) NXR_1108 Description: Ubiquitous expression, floxed RFP GFP reporter. Ubiquitously expressed RFP; upon cre-medmiated recombination expresses GFP and loses RFP. Reference: Transgenic generated by Bettina Ryll.听 Xtr.Tg(Ntub GalPR BicB) NXR_1109 Description: (Ntub GalPR BicB) Ntub Galpr Bic- N-tubulin promoter driving Gal4Pr (RU482-inducible Gal4-progesterone receptor VP16 fusion) in post-mitotic neurons. Cry-CFP marker cassette (linked) 搁别蹿别谤别苍肠别:听 Xtr.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP)Amaya NXR_1110 Description: (UbiqGal4,cryRFP) Human Ubiquitin C (UBC) promoter driving Gal4-VP16 fusion construct, with crystallin CFP marker. 搁别蹿别谤别苍肠别:听听 Xtr.Tg(Dre.mxn1:GFP)Dorey NXR_1111 Description: (zHB9-GFP) Zebrafish HB9 promoter driving GFP. GFP expression in motorneurons and kidney. 搁别蹿别谤别苍肠别:听听 Xtr.Tg(UAS:msn-mCherry;cryga:RFP)Amaya NXR_1117 Description: (UASmCherry-Moesin) UUAS driving mCherry-Moesin, 纬-cry driving RFP marker. Labels F-actin. 搁别蹿别谤别苍肠别:听听 Xtr.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR NXR_1123 Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas Reference: Custom transgenic generated by the NXR.